Fidelity of DNA Polymerase α Partially Purified from a Mutator Mutant and Wild-Type Mouse FM3A Cells
スポンサーリンク
概要
- 論文の詳細を見る
Several mutator mutants were isolated from a cultured mouse cell line (FM3A). The mutants exhibited a high rate of spontaneous mutation at three genetic loci of drug resistance. To investigate a possible link between mutator phenotype and fidelity of DNA replication, DNA polymerase α was partially purified from the wild-type and the mutator mutant (Fmut 1) showing the highest mutation rate. Using a combination of synthetic template and primer, the ratio of incorporation of incorrect to correct nucleotides was determined. The results indicated that the DNA polymerase α from the mutator mutant showed a slightly higher rate of misincorporation, 1.4 and 1.6 times, than that of the wild-type.
- 東海大学の論文
著者
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Hyodo Masao
Department of Biological Science and Technology, Tokai University School of High-technology for Huma
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SUZUKI Kenshi
Department of Hematology, Japanese Red Cross Medical Center Hospital
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Hyodo Masao
Department Of Biological Science And Technology Tokai University
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Suzuki K
Tokai Univ.
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Suzuki Kenshi
Department Of Hematology Japanese Red Cross Medical Center
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