Inhibition in Maturation of Nascent DNA Fragments in Cultured Mouse FM3A Cells after Treatment with DNA-Damaging Agents
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概要
- 論文の詳細を見る
The effect of DNA-crosslinking agents (cisplatin and mitomycin C), a DNA-intercalating agent (adriamycin) or mono functional psoralen (4-Met-4', 5'-dihydropsoralen plus near-ultraviolet radiation) on DNA replication in cultured mouse FM3A cells was studied by sedimentaion of the pulse-labeled DNA in an alkaline sucrose gradient. There was no inhibition of the maturation process of the nascent DNA 1 h after treatment of the cells with cisplatin, mitomycin C or adriamycin. However, this process was inhibited progressively during further incubation for 4 to 16 h without the agents, and accumulation of the nascent DNA fragments was observed. In the case of DNA-crosslinking agents, this inhibition may be correlated with an increase in the number of DNA-crosslinks formed during incubation. However, direct inhibition of maturation was not likely since the number was small, up to 4.9 per 10^9 Da. After treatment of cells with mono functional psoralen, no inhibition of maturation of the nascent DNA fragments was observed. In all cases, there was no degradation of DNA during incubation.
- 東海大学の論文
著者
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Hyodo Masao
Department of Biological Science and Technology, Tokai University School of High-technology for Huma
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SUZUKI Kenshi
Department of Hematology, Japanese Red Cross Medical Center Hospital
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Suzuki Kenshi
東海大学 分子生物
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Hyodo M
Tokai Univ.
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Hyodo Masao
Department Of Biological Science And Technology Tokai University
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YAMASHITA Midori
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Suzuki K
Tokai Univ.
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Suzuki Kenshi
Department Of Hematology Japanese Red Cross Medical Center
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Yamashita Midori
Department Of Molecular Biology School Of Medicine Tokai University
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