培養細胞の増殖におよぼすレーザーの出力とエネルギー密度に関する研究

概要

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Low-power laser irradiation on oral tissue is reported to have healing effects caused by accelerating cell growth. However, the effects of high-power laser irradiation on cell growth are unknown. To investigate the effects of high-power laser irradiation on oral tissue, we examined the cell growth of fibroblasts (Balb/3T3(clone)A31), osteoblasts (MC3T3-E1) and epithelial cells (normal human gingival keratinocytes) after laser irradiation in vitro using MTT assay. Cells were cultured during 1 day following laser irradiation once for 30, 60, 90, 120 and 180 seconds with a diode laser (830±10nm, GaAlAs diode, continuous wave, 400mW or 800mW). Non-irradiation cells were used as the control group. As a result, fibroblasts and epithelial cells showed good cell growth under the irradiation of 3.7mW/cm^2 for 90seconds. The irradiation of 3.7mW/cm^2 for 90seconds and of 6.7mW/cm^2 for 60seconds accelerated the cell growth of osteoblasts. These results suggest that laser irradiation of 3.7mW/cm^2 for 90seconds accelerates cell growth. In addition, power density (mW/cm^2) and irradiation time (seconds) are more closely related to cell growth in vitro than to energy density (J/cm^2).
奥羽大学の論文
2003-12-30