NIH Imageのマクロによる共焦点レーザー顕微鏡の画像解析

概要

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Digital images of confocal laser scanning microscope were analyzed by using several macro commands with NIH Image software to reveal the influence of He-Ne laser irradiation on the cytoplasmic organelles in MC3T3-E1 osteogenic cells. The mitochondria were stained by commercial fluorescent probes, named MitoTracker^<TM> Green FM and captured with Nikon PCM2000 confocal laser scanning microscope. Then, these mitochondrial images were analyzed by using macro commands such as stack, center point, summed image, protrusion and retraction. In this paper, it is discussed how exactly to obtain the suitable conditions for processing digital images, such as upper/lower points of a look up table (LUT), range of density slices and smoothing treatment. Furthermore, we recognize that the macro commands with NIH Image software are very useful for analyzing sequential images automatically.
奥羽大学の論文