表計算シートを活用する競合型ラジオイムノアッセイのシミュレーション

概要

論文の詳細を見る
Worksheet templates have been prepared for use in developing novel immunoassay systems. One is for drawing Scatchard plots to calculate the affinity constant (Ka) from experimental data by least squares regression. The other is to construct the theoretical standard curve in competitive radioimmunoassay (RIA) using the dissociation constant (Kd=1/Ka). The former is useful to characterize antibodies for screening. The latter can be used to estimate the theoretical detection limit of competitive RIA, for comparison in assessment of developing noncompetitive hapten immunoassay system. The evaluation of these worksheets was carried out by applying them to actual RIA data, comparing theoretical and observed standard curves. The values were in fair agreement with each other, using polyclonal and monoclonal antibodies, and single-chain Fv fragments. As spreadsheet programs are popular in personal computers, they are useful for the purpose of characterizing antibodies and assessment of assay systems.
東北大学の論文
2005-07-31