<Original article>Serum Soluble Fas as a Seromarker of Disease Activity in Chronic Hepatitis C and the Possible Involvement of Peripheral Blood Mononuclear Cells in the Production of Serum Soluble Fas
スポンサーリンク
概要
- 論文の詳細を見る
Fas-mediated apoptosis is considered to be a major form of liver cell death in chronic hepatitis C. Recently, serum soluble Fas has become measurable although its clinical significance in chronic liver diseases is not fully understood. We measured serum soluble Fas (sFas) levels in 22 normal volunteers, in 10 cases with autoimmune hepatitis type 1 (AIH), in 18 cases with chronic hepatitis C with autoimmune features (C-AIH), in 34 cases with chronic hepatitis without such features (C-CH) and in 17 cases with systemic lupus erythematosus. The mean sFas level in C-AIH was significantly higher (p<0.05) than C-CH and normal volunteers. As a whole, the serum sFas level positively correlated with aspartate aminotransferase (AST) (p<0.05) and alanine aminotransferase (ALT) (p<0.05), and inversely with peripheral platelet counts (p<0.05). Only C-CH showed a striking correlation between serum sFas and AST/ALT levels while other disease groups with autoimmune features did not, suggesting the possible involvement of lymphocytes in sFas production in these groups. The serum sFas level also significantly correlated with the histological activity indexes of chronic inflammation in liver sections. Fas and sFas mRNAs were both detectable by reverse-transcription-polymerase chain reaction amplification in peripheral blood mononuclear cells (PBMC) that were collected from these patients. The intensity of sFas mRNA expression was higher than that of full-length Fas mRNA in half of the cases examined. Among these, the expression intensity of both mRNAs was highest in AIH. After the stimulation of PBMC with phytohemoagglutinin and interleukin-2, the intensity of Fas mRNA expression was consistently higher than that of sFas mRNA. The Fas and sFas mRNA expressions in 3 C-CH liver samples were both more up-regulated than in the normal liver. Nuclear DNA fragmentation visualized by terminal deoxyuridine transferase-mediated nick end labeling was detectable in mononuclear cells infiltrating portal triads of the AIH, C-AIH and C-CH liver. In conclusion, serum sFas levels can be a possible seromarker of disease activity in chronic hepatitis C. Moreover, peripheral blood mononuclear cells are possibly involved in the production of serum sFas, especially in chronic hepatitis C with autoimmune features.
- 新潟大学の論文
著者
-
TAKAHASHI Toru
Division of Hematology, Department of Medicine, Yamaguchi Prefecture Central Hospital
-
Kenichi WATANABE
Department of Clinical Pharmacology, Niigata College of Pharmacy
-
Takahashi T
Division Of Gastroenterology And Hepatology Department Of Cellular Function Graduate School Of Medic
-
Asakura H
Niigata Univ. Graduate School Of Medical And Dental Sci. Niigata Jpn
-
Asakura Hitoshi
Division Of Gastroenterology And Hepatology Department Of Cellular Function Department Of Molecular
-
Asakura Hitoshi
Division Of Hepatology And Gastroenterology Niigata University Graduate School Of Medical And Dental
-
WATANABE Koji
Division of General Surgery, National Center for Child Health and Development
-
BOKU Saihiro
Division of Hepatology and Gastroenterology, Niigata University Graduate School of Medical and Denta
-
Boku Saihiro
Division Of Hepatology And Gastroenterology Niigata University Graduate School Of Medical And Dental
-
Watanabe Koji
Division Of General Surgery National Center For Child Health And Development
-
Watanabe Koji
Division Of Hepatology And Gastroenterology Niigata University Graduate School Of Medical And Dental
-
Takahashi Toru
Division Of Diabetes And Metabolism Department Of Internal Medicine Iwate Medical University School
-
Takahashi Toru
Division Of Hepatology And Gastroenterology Niigata University Graduate School Of Medical And Dental
-
WATANABE Koji
Division of Developmental Stomatognathic Function Science, Department of Growth and Development of Functions, Kyushu Dental College
関連論文
- Morphological and Cytogenetic Changes in Therapy-Related Leukemia Developed in a t(8;21)-Acute Myeloid Leukemia (M2) Patient: Sequential Cytogenetic and Molecular Analyses
- Preserved Lusitropic Reserve of the Left Ventricles in Spite ofImpaired Inotropic Reserve in Patients with Chronic Heart Failure
- Three-Dimensional Imaging of the Radiological StereoscopicVisualization of the Pancreatic Duct System by Endoscopic RetrogradePancreatography-Helical Computed Tomography
- Heterogeneity of p53 Mutational Status in Intramucosal Carcinoma of the Colorectum
- APC, K-ras codon 12 mutations and p53 gene expression in carcinoma and adenoma of the gall-bladder suggest two genetic pathways in gall-bladder carcinogenesis
- A Study of Patient with Chronic Non-B, Non-C Hepatitis who Developed Liver Cirrhosis and Hepatocellular Carcinoma Over 14 Years Observation
- Effects of Cisapride and Protective Effects of Mexiletine on the QTInterval in Rats
- Fine Structure of the Mouse Portal Vein in Relation to Its Peristaltic Movement
- The Effect and Mechanism of 5-Aminosalicyclic Acid Enema Treatment on Experimental Colitis Induced by a Murine Retrovirus
- A CASE WITH DIFFUSE NARROWING OF THE MAIN PANCREATIC DUCT SUCCESSFULLY TREATED BY STEROID THERAPY
- Microheterogeneity analysis of serum transferrin before and after after therapies to hepatocellular carcinoma
- Kinetic Analysis of Phenotypes of Cells and Their Cytokine Expression in the Development of Colitis in Nude Mice with MAIDS Colitis
- Liver metastasis 30 years after enucleation of the left eyeball for choroidal melanoma
- Late Phase Images in Whole Liver Double Phase CT during Hepatic Arteriography in the Diagnosis of Well-differentiated Hepatocellular Carcinomas
- Chemical Deafferentation of the Nasal Neuroepithelium Profoundly Reduces Cholecystokinin Gene Expression in the Mouse Olfactory Bulb
- Efficacy of a Flow Cytometry Method for the Detection of an Anti-neutrophil Cytoplasmi Antibody in Ulcerative Colitis: A Comparison with an Indirect Immunofluorescence Method
- The fucosylation index of serum α-fetoprotein as useful prognostic factor in patients with hepatocellular carcinoma in special reference to chronological changes
- Abrupt Duodenal Obstruction Caused by Traumatic Intramural Hematoma
- A Pregnant Case of Severe Acute Hepatitis Type C Successfully Treatedwith Natural Interferon-Alpha and Showing No Evidence of a MaternalTransmission of HCV to the Newborn
- High dose intravenous methylprednisolone resolves esophageal stricture resistant to balloon dilatation with intralesional injection of dexamethasone
- Ectopic ACTH Syndrome Due to Thymic Atypical Carcinoid Treated with Combination Chemotherapy of Cisplatin and Etoposide
- Alterations in Cardiac β-Adrenoceptors During the Development of RatExperimental Autoimmune Myocarditis
- Effect of Regular Exercise on Coronary Endothelial Function in Patients With Recent Myocardial Infarction
- Higher Serum Free Triiodothyronine Levels within the Normal Range Are Associated with Metabolic Syndrome Components in Type 2 Diabetic Subjects with Euthyroidism
- Serum Soluble Fas as a Seromarker of Disease Activity in Chronic Hepatitis C and the Possible Involvement of Peripheral Blood Mononuclear Cells in the Production of Serum Soluble Fas
- Rice Cake Ileus - A Rare and Ethnic but Important Disease Status in East-Southern Asia
- Different Susceptibility to Insulin Resistance and Fatty Liver Depending on the Combination of TNF-α C-857T and Adiponectin G+276T Gene Polymorphisms in Japanese Subjects with Type 2 Diabetes
- Influence of childhood type II diabetes on bone formation in the growth period