Efficacy of a Flow Cytometry Method for the Detection of an Anti-neutrophil Cytoplasmi Antibody in Ulcerative Colitis: A Comparison with an Indirect Immunofluorescence Method
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概要
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Purpose : The anti-neutrophil cytoplasmic antibody (ANCA) in the serum of patients with ulcerative colitis (UC) can serve as a serological tool for making differential diagnosis from Crohn's disease (CD) and indicating a higher relapse rate in UC. This study was performed to clarify the clinical efficacy of the flow cytometry method for detecting ANCA in the serum of patients with UC, comparing this with an indirect immunofluorescence microscopy (IIF). Materials and Methods : Forty-two sera from 21 patients with active and inactive UC and seven sera from 7 patients with CD were obtained. Flow cytometry and IIF methods were employed to detect ANCA. For flow cytometry, indicator neutrophils were permeabilized by Saponin treatment, resuspended in the diluted sera, and incubated in FITC-conjugated goat antihuman immunoglobulin. These cells were analysed on a flow cytometer. For IIF, a commercial kit to detect ANCA was used. Results : Flow cytometry showed ANCA positivity in thirty-five of the 42 sera tested, and IIF in twenty-eight of the 42 sera tested. When compared with IIF, the flow cytometry for ANCA had a sensitivity of 96.5% and a specificity of 63.6%. ANCA positivity by flow cytometry and by IIF appeared in 23 and 19 of 28 sera tested in the entire colitis, respectively, and yielded almost similar results in left sided colitis. Positive rates and titers for ANCA by flow cytometry in the chronic continuous and relapse/remittent types of UC (intractable type) were significantly higher than those of the initial attack type of UC (non-intractable) and CD. Conclusion : ANCA positivity by flow cytometry was higher than that by IIF. The flow cytometry method is as clinically useful as the IIF method for detecting ANCA in UC because it is assessed objectively and quantitatively.
- 新潟大学の論文
著者
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Hasegawa K
Department Of Orthopaedic Surgery Niigata University School Of Medicine
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Asakura H
Niigata Univ. Graduate School Of Medical And Dental Sci. Niigata Jpn
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SATOH Sadayuki
The 3^rd Department of Internal Medicine, Niigata University School of Medicine
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ASAKURA Hitoshi
The 3^rd Department of Internal Medicine, Niigata University School of Medicine
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HASEGAWA Katsuhiko
The 3^rd Department of Internal Medicine, Niigata University School of Medicine
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Asakura Hitoshi
Division Of Hepatology And Gastroenterology Niigata University Graduate School Of Medical And Dental
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Satoh Sadayuki
The 3^rd Department Of Internal Medicine Niigata University School Of Medicine
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Hasegawa K
Niigata Univ. Hospital Jpn
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Sugimura Kazuhito
Division Of Gastroenterology And Hepatology Graduate School Of Medical And Dental Sciences Niigata U
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Suzuki Katsunori
新潟大学 医歯学総合研究科内分泌･代謝学分野
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