高速液体クロマトグラフィーによるオキシンー銅錯体の解離反応速度定数の測定

概要

論文の詳細を見る
The solvolytic dissociation kinetics of the oxine-copper complex {bis (8-quinolinolato) copper (II)} was studied with a reversed-phase high-performance liquid chromatography and spectrophotometry. The analysis of the chromatographic peak height of the complex, which decreased with a decrement of the flow rate of the eluent {water-acetonitrile (70:30 wt%)}, gave an apparent rate constant k_d of 1.4±0.5×10^<-4> s^<-1> for the dissociation reaction. With the spectrophotometric method, the apparent rate constant k_<ex> of the ligand exchange with EDTA was determined to be 0.75±0.11 s^<-1>. The observed small value of k_d, compared to the value of k_<ex>, can be attributed to the difference of mechanisms between the ligand-exchange reaction and the solvolytic dissociation. The value of k_<ex> is controlled by the concentration of EDTA, indicating that EDTA was involved with the rate-determining step of the ligand-exchange reaction. On the other hand, the solvolytic dissociation of the oxine-copper complex is induced by a lack of 8-quinolinol around complex.
社団法人日本分析化学会の論文
2006-03-05