唾液および血清中のHistamine分離定量法について

概要

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The histamine contents in whole saliva and serum collected from patients with periodontal disease were measured by both Anton-Sayre's and Hakanson-Ronnberg's methods. Comparative study of both methods was performed to find out the better way of estimating histamine. The following results were obtained : 1. Compared with Anton-Sayre's method which was able to measure histamine in the range of concentrations from 0.05 to 10.0μg/ml, Hakanson-Ronnberg's method was much sensitive to detect from 2 to 1,000μg/ml. 2. The recovery of histamine was 70.0% by Anton-Sayre's method and 92.2% by Hakanson-Ronnberg's method. 3. Both methods were capable of measuring of histamine contents in whole saliva and serum of all subjects ewamined. 4. The histamine cotents in whole saliva was distinctly higher in patients with periodontal disease than clinically health subjects. 5. There was no difference in the serum histamine contents between clinically healthy subjects and periodontal disease patients.
特定非営利活動法人日本歯周病学会の論文
1976-09-28