仮性結核菌(Yersinia pseudotuberculosis)に関する研究 : III. 分離培養について

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The distribution of Yersinia pseuclotuberculosis in man and many species of animalsvaries geographically. The frequency of isolation of this organism is much lower inJapan than in European countries. In their previous report19), the authors stressedthat full investigation should be performed to determine the distribution of Y. pseudo-tuberculosis in Japan. It is necessary to examine the organism recovered from feces inorder to clarify the distribution and the epizootiology of the organism. It is known thatthe isolation of Y. pseudotuberculosis from contaminated materials is difficult. Nosatisfactory method has been established to isolate Y. pseudotuberculosis from suchcontaminated materials as feces,In the present study, the authors examined the selective medium and the enrichmentculture method described by PATFIRSON a-nd COOKS). The results obtained are asfollows.l. Using the enrichment culture method, the authors confirmed PATERSON andCOOKS findings that Y. pseudotuberculosis multiplied gradually to reach a maximumlevel of growth after 20 days, provided that the specimen was a 10 per cent suspensionin M/15 phosphate buffer solution (pH 7.6) and the culture maintained at 5C.2. Y. pseudotuberculosis exhibited better growth on MacConkeys agar than on theselective medium described by PATE,RSON and COOK. It was considered that MacConkeysagar might be suitable as the selective medium for this organism.3. Satisfactory results were obtained on the recovery of Y. pseudotuberculosis froman artificial mixture of feces and from the feces of experimentally infected guinea pigs andnaturally infected rabbits when the following method had been used: (a) The specimenwas a 10 per cent suspension in phosphate buffer solution, (b) the culture was main-tained at 5C for 3 weeks, and (c) lMacConkeys agar was used for the isolation.4. It is considered that the same method as used for Y. pseudotuberculosis may beeffective for the isolation of Y. enterocolitica.
社団法人日本獣医学会の論文
1973-02-25

仮性結核菌(Yersinia pseudotuberculosis)に関する研究 : III. 分離培養について

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