Photoconversion of a Water-Soluble Chlorophyll Protein from Chenopodium Album : Resonance Raman and Fourier Transform Infrared Study of Protein and Pigment Structures
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概要
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A water-soluble Chl a/b-protein complex, CP668, from Chenopodium album converts to another form of protein complex, CP743, upon light illumination. Structural changes of pigments and proteins upon photoconversion were studied using resonance Raman (RR) and Fourier transform infrared (FTIR) spectroscopies. RR spectra of CP668 and CP743 and a light-induced FTIR difference spectrum showed that the macrocyle C=C bands of Chl a in CP668 considerably changed upon conversion to the pigment (not chemically identified yet) in CP743. The C=C band pattern of the RR spectrum of CP743 was similar to that of bacteriochlorophyll a, suggesting that the conjugated system of the CP743 pigment resembles a bacteriochlorin ring. Judging from the C=O frequencies, the 13^1-keto C=O groups of Chl a and b in CP668 are free from hydrogen bonding, whereas the 13^2-ester C=O groups of both Chl a and b and the 7-formyl C=O of Chl b in CP668 are hydrogen bonded. Upon conversion to CP743, interactions of the 13^1-keto and 13^2-ester C=O groups were basically unaffected, demonstrating no drastic changes around these C=O groups. FTIR spectra in the amide I' region of CP668 and CP743 in D_2O buffer showed a peak at 1, 633 cm^<-1> which represents a major component of β-sheet conformation. Second-derivative spectra of the amide I' bands as well as a light-induced FTIR difference spectrum suggested that drastic change in the protein conformation does not occur upon photoconversion.
- 日本植物生理学会の論文
著者
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Inoue Yorinao
Photosynthesis Research Laboratory The Inst. Of Phys. And Chem. Res. (riken)
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Noguchi Takumi
Photosynth. Res. Lab. Inst. Phys. Chem. Res. (riken)
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Noguchi Takumi
Photosyn. Res. Lab. Inst. Phys. Chem.
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Noguchi Takumi
Photosynthesis Research Lab. The Institute Of Physical And Chemical Research (riken)
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Itoh Shigeru
National Institute for Basic Biology
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Inoue Yorinao
Photosyn. Res. Lab. Inst. Phys. Chem.
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Inoue Yorinao
Photosynthesis Research Lab. The Institute Of Physical And Chemical Research (riken)
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Kamimura Yasumaro
Department of Biology, Faculty of Science, Toho University
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Kamimura Yasumaro
Department Of Biology Faculty Of General Education Toho University
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Kamimura Yasumaro
Department Of Biology Faculty Of Science Toho University
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Inoue Yorinao
Photoshnthesis Research Laboratory The Institute Of Physical Anc Chemical Research (riken)
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NOGUCHI Takumi
Photosyn. Res. Lab. , The Inst. Phys. Chem. Res. (RIKEN)
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NOGUCHI Takumi
Photosyn. Res. Lab. , The Inst. of Phys. and Chem. Res. (RIKEN)
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