Characteristics of the Cl^- Action Site In the O_2 Evolving Reaction in PS II ParticleS : Electrostatic Interaction with Ions
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概要
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The role of Cl^- in the reactivation of O_2 evolution in photosystem II (PS II) particles derived from spinach chloroplasts was studied in the presence of various salts. Multi-valent ion (especially anion) salts were found to strongly suppress the reactivation of O_2 evolution by Cl^- in the Cl^--depleted PS II particles in a competitive manner. The effectiveness of anions in the suppression of Cl^--supported O_2 evolution was in the order of trivalent < divalent < monovalent ones. Multivalent anions similarly suppressed O_2 evolution in the untreated PS II particles under low and moderate Cl^-- concentrations. pH dependence of the Cl^--affinity (K_m) value for Cl^-) was also studied. Within the pH range 5.5to 8 the K_m value became higher as the pH of the medium increased. These results suggest that the membrane surface in the vicinity of the Cl^- action site is net positively charged and attracts Cl^- electrostatically, and that the site is almost freely accessible to various anions. The origin and role of the local net positive domain and the role of peripheral proteins are discussed.
- 日本植物生理学会の論文
- 1986-01-00
著者
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Itoh Shigeru
National Institute for Basic Biology
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Uwano Sachiko
National Institute for Basic Biology
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