Reconstitution of the Photosystem I Secondary Quinone Acceptor (A_1) in the P700-F?_x Core Isolated from Synechococcus PCC 6301

概要

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By treating a F_A/F_B-depleted P700-F_x core from Synechococcus PCC 6301 with diethylether, most of the phylloquinone was removed without loss of P700. The 1 ms decay of P700^+ in the original core was replaced by the 25 ns decay, which was interpreted as the backreaction occurring in a P700^+-F_x^- pair in place of a P700+_F~ pair. When phylloquinone was added to the phylloquinone-depleted core, the 1 ms decay of P700^+ was recovered only in a concomitant presence of Triton X-100. The effective concentration of Triton X-100 was between 0.02% and 0.1%. The flash-induced difference spectrum from 400 to 500 nm confirms the participation of F_x in the 1 ms decay of P700^+ in the reconstituted core. Washing the phylloquinone-reconstituted core with n-hexane did not eliminate the effects of phylloquinone, suggesting that the reconstituted phylloquinone was as tightly bound to the P700-F_x core as is the naturally-occurring A_1.
日本植物生理学会の論文