Presence of the Photoactive Reaction-Center Chlorophyll of PSI (P700) in Dark-Grown Cells of a Chlorophyll-Deficient Mutant of Chlorella kessleri

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Compositions of pigments and polypeptides of pale green membranes that had been isolated from dark-grown cells of a chlorophyll-deficient mutant of Chlorella kessleri were investigated. They contained Chl a in a level corresponding to about 1% of that present in the thylakoid membranes isolated from autotrophically grown wild-type cells and a trace amount of chlorophyllide a, but neither Chl b nor carotenoids. The polypeptide profile of the mutant membranes was similar to that of membranes isolated from wild-type cells that were grown in the dark. Neither the chlorophyll-binding subunits of PSI nor the apoproteins of LHCP were detected by SDS-PAGE and immunoblot analysis. However, the light-minus-dark difference spectrum of the mutant membranes revealed the presence of the reaction-center chlorophyll of PSI (P700) at a molar ratio of 190 chlorophyll (Chl a plus Chlide a) per P700. P700 was more stable than Chl a and Chlide a in the light so that prolonged illumination led to a decline in the Chl/P700 ratio to 24. The initial rate of P700 photooxidation in the mutant membranes was comparable to that in CP1 isolated from the dark-grown wild-type cells. Under illumination with strong light, the initial rate was decreased in parallel to the decrease in Chl/P700 ratio. The results suggest that most of Chl present in the mutant membranes can transfer excitation energy to P700.
日本植物生理学会の論文