Purification and Properties of NAD-Dependent Sorbitol Dehydrogenase from Apple Fruit : PROTEINS, ENZYMES AND METABOLISM
スポンサーリンク
概要
- 論文の詳細を見る
This is the first report of the purification of NAD-dependent sorbitol dehydrogenase (NAD-SDH) from a plant source. The enzyme was extracted from apple (Malus domestica cv. Ourin) fruit and purified until it appeared as a single polypeptide chain on a gel after SDS-PAGE. From the apparent molecular mass of 62 kDa obtained by SDS-PAGE and that of 120 kDa by gel filtration, the enzyme appeared to be a homodimer. Maximum rates of oxidation of sorbitol and reduction of fructose were observed at pH 9.6 and pH 6.0, respectively. The K_m for oxidation of sorbitol was 40.3 mM and that for reduction of fructose was 215 mM. The maximum rate of oxidation of sorbitol was about 10 times higher than that of the reduction of fructose. The results of the kinetic analysis strongly suggest that in vivo the enzyme would favor the conversion of sorbitol to fructose over the reverse reaction. None of the divalent cations tested had any effect on the oxidation of sorbitol by NAD-SDH. The reaction catalyzed by NAD-SDH was not specific to sorbitol and other substrates could also be oxidized. Among the tested substrates, ethyl alcohol had a particularly high affinity for the enzyme.
- 日本植物生理学会の論文
著者
-
Yamaki Shohei
Laboratory Of Horticultural Science Graduate School Of Bioagricultural Sciences Nagoya University
-
Kanayama Yoshinori
Laboratory of Horticultural Science, School of Agricultural Sciences, Nagoya University
-
Uamaguchi Hideaki
Laboratory of Horticultural Science, School of Agricultural Sciences, Nagoya University
-
Yamaki Shohei
Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University
-
Uamaguchi Hideaki
Laboratory Of Horticultural Science School Of Agricultural Sciences Nagoya University
-
Kanayama Yoshinori
Laboratory Of Horticultural Science School Of Agricultural Sciences Nagoya University
関連論文
- Analyses of Indole Acetic Acid and Abscisic Acid Contents in Nodules of Soybean Plants Bearing VA Mycorrhizas
- Molecular Cloning of Vacuolar H^+-pyrophosphatase and Its Expression during the Development of Pear Fruit
- Changes in H^+-Pumps and a Tonoplast Intrinsic Protein of Vacuolar Membranes during the Development of Pear Fruit
- Characterization of Hexose Transporter for Facilitated Diffusion of the Tonoplast Vesicles from Pear Fruit
- PROPERTIES AND ROLES IN FRUIT DEVELOPMENTOF VACUOLAR H^+-ATPASE, H H^+-PYROPHOSPHATASE ANDVM-23 IN PEAR FRUIT
- Purification and Properties of NADP-Dependent Sorbitol-6-Phosphate Dehydrogenase from Apple Seedlings
- Purification and Characterization of Two Sucrose Synthase Isoforms from Japanese Pear Fruit
- Purification and Properties of NAD-Dependent Sorbitol Dehydrogenase from Apple Fruit : PROTEINS, ENZYMES AND METABOLISM
- Stimulation of the Uptake of Sorbitol into Vacuoles from Apple Fruit Flesh by Abscisic Acid and into Protoplasts by Indoleacetic Acid
- The mechanism of chilling injury in sweet potato VI. Changes of lipid components in the mitochondrial membrane during chilling storage
- Mechanism of chilling injury in sweet potato VII. Changes in mitochondrial structure during chilling storage
- Mechanism of chilling injury in sweet potato XI. Irreversibility of physiological deterioration
- Mechanism of chilling injury in sweet potato Part XII. Temperature dependency of succinoxidase activity and lipid-protein interaction in mitochondria from healthy or chilling-stored tissue
- Sugar Metabolic Process of the ^C-photoassimilates on the Translocation Pathways of Tomato Plants(Plant Production Science Horicultural Science)
- Critical Photoperiod for Flower Bud Initiation in Everbearing Strawberry 'Summerberry' Plants Grown at High Temperatures(Plant Production Science, Horticultural Science)
- Biochemistry and Genetic Engineering of Sugar Metabolism in Fruit