Boron Nutrition of Cultured Tobacco BY-2 Cells : I. Requirement for and Intracellular Localization of Boron and Selection of Cells that Tolerate Low Levels of Boron : GROWTH AND DEVELOPMENT : ENVIRONMENTAL AND STRESS RESPONSES

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Cultured cells of tobacco (Nicotiana tabacum L. cv. Bright Yellow 2) grown under the standard culture conditions (1 mg boron liter^<-1> medium as boric acid) contained boron at a concentration of 2.26 mg boron kg^<-1> oven-dried cells and the protoplast contained 1.26% of the boron in the cells. The cells required boron for growth and the half-maximum growth rate was obtained with 0.056 mg of boron liter^<-1> medium. Subculturing the cells in media with lower concentrations of boron allowed selection of cells that can grow even in the presence of 1 μg boron liter^<-1> medium. Cell walls of the selected cells seemed to be thicker than those of the control cells and Golgi bodies were accompanied by more secretory vesicles than those in the control cells.
日本植物生理学会の論文