Characteristics of the Inhibitory Effect of 5-Fluorodeoxyuridine on Cytodifferentiation into Tracheary Elements of Isolated Mesophyll Cells of Zinnia elegans

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概要

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• The inhibitory effect of 5-fluorodeoxyuridine (FdU) on the differentiation into tracheary elements was characterized in isolated mesophyll cells of Zinnia elegans. Both thymidine and uridine counteracted the inhibitory effect of FdU on the differentiation into tracheary elements, while only thymidine was effective in counteracting the effect of FdU on cell division. Higher concentrations of thymidine were needed for the restoration of the differentiation that was blocked by FdU than for the restoration of cell division. These results suggest that FdU prevents the differentiation via a mechanism that is different from the inhibition of thymidylate (dTMP) synthase by fluorodeoxyuridine monophosphate (FdUMP), derived from FdU, to which the blockage of cell division by FdU should be attributable. The differentiation into tracheary elements was prevented when FdU was added earlier than the 36th hour of culture, and thymidine overcame the inhibitory effect of FdU only when added within the first 4 h of culture. Pretreatment with FdU before application of 6-benzyladenine (BA) and 1-naphthaleneacetic acid (NAA), which are essential for the formation of tracheary elements, also inhibited the differentiation. Thus, the aspect of the differentiation that is the target of inhibition by FdU appears to occur between the 4th hour and the 36th hour of culture and to begin even in the absence of exogenous plant growth regulators.

• 日本植物生理学会の論文

著者

• Fukuda H

  Univ. Tokyo Tokyo Jpn

• Sugiyama Munetaka

  Biological Institute Graduate School Of Science Tohoku University:(present)botanical Gardens Faculty

• Sugiyama Munetaka

  Biological Institute Faculty Of Science Tohoku University

• Sugiyama Munetaka

  Botanical Gardens Graduate School Of Science University Of Tokyo

• Sugiyama M

  Biological Institute Graduate School Of Science Tohoku University:(present)botanical Gardens Faculty

• Sugiyama M

  Botanical Gardens Graduate School Of Science The University Of Tokyo

• FUKUDA Hiroo

  Biological Institute, Faculty of Science, Tohoku University

• Komamine A

  The Research Institute Of Evolutionary Biology

• Komamine Atsushi

  Biological Institute Faculty Of Science Tohoku University

• Fukuda Hiroo

  Biological Institute Faculty Of Science Tohoku University

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