Purification and Characterization of a Mitochondrial DNA Polymerase from Cultured Tobacco Cells

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概要

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• The mitochondrial DNA polymerase of tobacco BY-2 cells was purified more than 1,200-fold by column chromatography on DEAE cellulose, phosphocellulose, and Affi-Prep heparin. The enzyme was classified as a γ-type DNA polymerase, in view of the inhibition of its activity by N-ethylmaleimide and dideoxy TTP, the absence of inhibition by aphidicolin and arabinosyl CTP, the stimulation by KCl, and the ability of the enzyme to utilize poly(A)-(dT)_<12-18> in the presence of Mn^<2+> ions. The molecular mass of the native mitochondrial DNA polymerase was estimat-ed to be 70-110 kDa by column chromatography on Superose 12. When DNA polymerase activity was analyzed after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the activity that polymerized DNA was observed as a single band of a protein with a molecular mass of approximately 110 kDa. Therefore, the tobacco mitochondrial DNA polymerase appears to consist of a single subunit of 110 kDa.

• 日本植物生理学会の論文

著者

• FUKUDA Hiroo

  Biological Institute, Faculty of Science, Tohoku University

• Sato Kumi

  Biological Institute Faculty Of Science Tohoku University:(present)botanical Gardens Faculty Of Scie

• Fukuda Hiroo

  Biological Institute Faculty Of Science Tohoku University

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