Conversion of L-Ascorbic Acid to L-Idonic Acid, L-Idono-γ-lactone ane 2-Keto-L-idonic Acid in Slices of Immature Grapes
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概要
- 論文の詳細を見る
L-[1-^<14>C]Ascorbic acid ([1-^<14>C]AA) was vacuum-infiltrated into slices of immature grapes (Vitis labrusca L., cv. "Delaware") after which incorporation of ^<14>C into each metabolite was investigated. Under the experimental conditions used, the metabolic reaction of AA proceeded at a constant rate within the reaction period (5 hr). As a result, ^<14>C first appeared in three metabolic products; L-idonic acid, L-idono-γ-lactone and 2-keto-L-idonic acid ( =2-keto-L-gulonic acid), after which incorporation of ^<14>C into tartaric acid (TA) took place. When slices of grapes were treated with iodoacetic acid, the incorporation of ^<14>C into TA completely stopped and in the inhibitor experiment, the amount of ^<14>C which had been incorporated into TA in the control was found to be divided among L-idonic acid, L-idono-γ-lactone and 2-keto-L-idonic acid. These results are strong evidence that at least one of these three compounds is the effective precursor of TA and is located on the metabolic pathway between AA and TA in the grape.
- 日本植物生理学会の論文
著者
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KASAI ZENZABURO
The Research Institute for Food Science, Kyoto University
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Kasai Zenzaburo
The Res Arch Instrtute For Food Sclence Kyoto University
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Saito Kazumi
The Radioisotope Research Center, Kyoto University
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Saito Kazumi
The Radioisotope Research Center. Kyoto University
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