Cell membrane permeability and respiratory activity in chilling stressed callus

概要

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To improve our understanding of the mechanism of chilling injury in chill-sensitive callus (Cornus stolomfera), early changes in cell permeability and respiratory activity were studied. Partial leakage of amino acids and an abrupt increase in permeation and oxidation of added dopamine were characteristic of chilled callus in the late stage of chilling at 0℃ (48 hr), when most of the callus sustained severe injury. However, little or no change in cell permeability was observed in the early stage of chilling (within 24 hr), when calli retained their viability for growth after transfer to a warm temperature. These results suggest that changes in the cell membranes per se are by no means the primary step in cell injury. Temporary depression of respiratory activity was detected soon after chilling for 12 hr, but activity appeared to return to the original level on further chilling up to 24 hr. An irreversible dysfunction, however, occurred in the respiratory system on prolonged chilling up to 48 hr. This implies that irreversible impairment of mitochondrial functions may not be involved in the early stage of the cell injury. A possible relationship between these observed changes arid ultrastructural changes in chilled cells is discussed.
日本植物生理学会の論文