Changes in nicotinamide nucleotide content and glucose metabolism in wheat embryos during vernalization

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Contents of nicotinamide nucleotides, NAD, NADP, NADH_2 and NADPH_2, in wheat embryos were determined during normal germination and cold treatment. They increased markedly in the early stage of germination then decreased later. During cold treatment, these nucleotides increased initially as in normal germination, but did not decrease in the later stages. The C_6/C_1 ratios during germination decreased gradually with aging, but after about the fourth day increased. This suggests that the pentose phosphate pathway operates actively. During cold treatment, the C_6/C_1 ratio decreased slightly with aging. This suggests that the EMP pathway operates predominantly in the glucose metabolism. Based on these results, the correlation between NADPH_2 and glucose metabolism during cold treatment was discussed. Embryos which had absorbed labelled glucose were fractionated into several chemical components. The radioisotope accumulated mainly in the sugar fraction, with small amounts in the organic acid, amino acid and nucleic acid fractions. Changes in the content of each component showed that sugar, organic acid and amino acid increased during cold treatment.
日本植物生理学会の論文