Studies on the vegetative life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture IV. Effects of 6-methyl purine on the revolution of the cell cycle

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Cells of Chlamydomonas reinhardi Dangeard were synchronously grown under a 12 hr light- 12 hr dark regime. The algal cell cycle under these conditions starts with a light-induced reaction(s) at the beginning of the light period and ends, after a definite period of time (23-24 hr at 25℃), in zoospore liberation. When cells were exposed to 6-methyl purine for short periods (0.5-2.5 hr) at different times during the early and intermediate phases of the cell cycle, it exerted, as an analogue of adenine, two different effects on the revolution of the cell cycle: one a "lengthening" effect seen at its low concentrations in which the length of the cell cycle was somewhat prolonged, the other a "return to start" effect at higher concentrations. In the latter a short exposure of cells to 6-methyl purine brought them to the starting point of the cell cycle concurrent with the abortion of the cycle in process. When 6-methyl purine was applied during the later phase of about 1/4 the length of the cell cycle, it casued no effect. Control of the revolution of the algal cell cycle by an "adenine-involving reaction(s)" disturbed by this adenine analogue is discussed.
日本植物生理学会の論文