Morphactin-kinetin interaction on growth and shoot formation in tobacco callus cultures
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概要
- 論文の詳細を見る
Pith-derived calluses of Nicotiana tabacum L. cv. Wisconsin no. 38 were inoculated on an RM-1964 medium containing various concentrations of a morphactin, chlor-flurenol (CFl) and kinetin (KIN). An addition of KIN (0.1-2 mg/liter) alone was effective for shoot formation from the calluses, but a high dose (10 mg/liter) resulted in the inhibition of growth and in no differentiation. The inhibitory effect of a high dose of KIN was counteracted with CFl. Three combinations of KIN and CFl; CFl (0.1 mg/liter)+KIN (2.0 mg/liter); CFl (0.1 mg/liter)+KIN (10.0 mg/liter) and CFl (1.0 mg/liter)+KIN (2.0 mg/liter), were successful for 100% shoot redifferentiation in inoculated calluses. An appropriate balance between CFl and KIN seems to be involved in shoot formation. The present results can best be interpreted by assuming that CFl acts as an auxin in cultured tissues.
- 日本植物生理学会の論文
著者
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Ogura Hisakazu
Laboratory Of Biology Faculty Of Education Okayama University
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Ogura Hisakazu
Laboratory Of Genetics Faculty Of Agriculture Kyoto University
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