Carotenoids and fatty alcohols as non-ionic hydrophobic inhibitors of photochemical activities chloroplasts
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概要
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Carotenoids and fatty alcohols were added to Intact spinach chloroplasts in suspension, and the effects of these reagents on the absorption spectrum and photochemical activities of chloroplasts were examined. The addition of lutein, β-carotene and neoxanthin transformed a chlorophyll a form (C684) having an absorption maximum at 684 nm into C666, and the activity of photosystem II decreased parallel with this transformation. The activity of photosystem I was completely unaffected by the effect of added carotenoids, whereas both cyclic and non-cyclic photophosphorylations were inhibited by the additions. These effects were reproducibly observed only with a "petroleum ether-soluble" fraction of each carotenoid, monomeric and less polymerized forms, which are soluble in petroleum ether at -25℃; the "insoluble" fraction of more polymerized forms was ineffective. Comparative experiments with lutein, DCMU and CCCP as inhibitors and the dependence of inhibition on light intensity suggested that the site of inhibition by carotenoids is on the water side of photosystem II between its reaction center and the CCCP-inhibiting site. Fatty alcohols with carbon atoms between 8 and 12 produced stronger but less specific effects on the spectrum and activities , At low concentrations the uncoupling of photophosphorylation occurred and, in a middle concentration range, the activity of photosystem II decreased with the transformation of C684 into C666 and with a change in carotenoid bands. At higher concentrations, where the enhanced activity of photosystem I decreased from a maximum to zero, the red band of chlorophyll a further changed in a complex manner and the absorption around J'~03 nm decreased. We, thus, deduced that C684 and some endogeneous. carotenoids are associated with photosystem II on its water side and that added carotenoids and fatty alcohols at lower concentrations change their states, resulting in the inhibition of this photosystem.
- 日本植物生理学会の論文
著者
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Shibata Kazuo
Laboratory Of Plant Physiology Institute Of Physical And Chemical Research (rikagaku Kenkyusho)
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Konishi Katsuhiko
Laboratory of Biological Chemistry, Tokyo Institute of Technology
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Yaginuma Nakatsugu
Laboratory of Biological Chemistry, Tokyo Institute of Technology
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Konishi Katsuhiko
Laboratory Of Biological Chemistry Tokyo Institute Of Technology
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Yaginuma Nakatsugu
Laboratory Of Biological Chemistry Tokyo Institute Of Technology
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