混合医薬品の定量分析研究(第1報) : Sodium 1,2-Naphthoquinone-4-sulfonateによるフェナセチンの比色定量法

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The separatory determination of phenacetine in some pharmaceutical formulae was examined utilizing coloration with sodium 1,2-naphthoquinone-4-sulfonate. The sample containing about 100 mg. of phenacetine was hydrolyzed by refluxing with 2 ml. of concentrated hydrochloric acid for 40 minutes. When cooled, the solution was transfered to a 200 ml. measuring flask, diluted to the mark with water, and mixed thoroughly. This solution was diluted farther with 5 volumes of water. To 2 ml. aliquot of this solution in Squibb's separatory funnel, 2 ml. of 2% potassium hydrogenphthalate and 0.7 ml. of 1% sodium 1,2-napathoquinone-4-sulfonate were added, the mixture was shaken for 3 minutes, and cooled on crushed ice for 3 minutes. Then the solution was extracted with 10 ml. of chloroform by shaking for 3 minutes. The extract was dried over anhydrous sodium sulfate in stoppered test tube. After 10 minutes, absorbance of the chloroform solution was measured at 485 mμ. The results obtained by this method are shown in Tables I and II.
公益社団法人日本薬学会の論文
1966-04-25