異性体分析(第3報) : D-グルコン酸塩とL-イドン酸塩およびD-アラボン酸塩とD-リボン酸塩の差別定量

概要

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Mixing ratio of a binary mixtures of D-gluconate and L-idonate, and of D-arabonate and D-ribonate, was determined from the measurement of optical rotation at 345 mμ, in the presence of a molybdate. In the case of D-gluconate and L-idonate, 5.0 m. mole of acetic acid and molybdate (Mo 0.5 mg. atom) were added to 0.05 m. mole of the prepared mixed sample, the whole was brought to 10.0 ml. with water, and optical rotation of the solution was measured at 345 mμ. There was a good linear relationship between mixing ratio and optical rotation. The same result was obtained with the mixture of D-arabonate and D-ribonate. Consequently, when the mixing ratio of a binary mixture of unknown composition containing aldonates is to be determined, the total amount of aldonates is first determined. exactly 0.05〜1.0 m. mole of the aldonates as a solution is measured, and the optical rotation of the solution is measured after addition of a molybdate. Ratio of aldonate mixed can be calculated from a calibration curve.
社団法人日本薬学会の論文
1966-11-25