血清Cl^-の螢光滴定
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A fluorometric method for titration of chloride based on Schales and Schales's mercurimetry is described. Anisidine Blue (tetrasodium o-dianisidine-N, N, N', N'-tetraacetate) is used as a fluorescent indicator. The blue fluorescence of the indicator is developed in the region above pH 2.5,and quenched with Hg^<2+> completely in the pH range of 3 to 4. Titration mixture is composed of 100μl of serum, 1000μl of Macllvaine buffer solution (pH 4), and 500μl of methanol solution of Anisidine Blue (1mg/ml). Titration is carried out under ultraviolet ray, with 0.02N Hg(NO_3)_2 easily until blue fluorescence is quenched. This method makes possible the direct titration of chloride in clinical materials such as icteric, hemolytic, and lipemic serum.
- 公益社団法人日本薬学会の論文
- 1976-08-25
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