Diphenylcarbazideを用いる血清遊離脂肪酸微量比色定量の改良について

概要

論文の詳細を見る
A method was developed for microdetermination of fatty acids in serum, based on the use of an improved copper reagent, without the addition of sodium chloride, for the lower phase and the extraction solvent as the upper phase for extraction of free fatty acid-copper complex. The improved copper reagent contains 10% (w/v) Cu (NO_3)_2・3H_2O, 2M triethanolamine, and 2M acetic acid in 10 : 9 : 1 (v/v) ratio in water and adjusted to pH 8.1. The extraction solvent is a 61 : 37 : 2 (v/v) mixture of heptane, chloroform, and methanol. After extraction, the upper phase containing the free fatty acid-copper soap is rapidly separated from the aqueous phase and free fatty acid is determined as a copper soap by colorimetry with 0.8% ethanol solution of diphenylcarbazide. This method was found to be about 3 times more sensitive than the conventional diethyldithiocarbamate method. It was also found that the color development is not affected by chain length of fatty acids in the range of C_<12> to C_<18>, but increases to some extent in the presence of lecithin. Sera from 29 patients were examined by this and the conventional method, and the correlation coefficient for the two methods was good.
公益社団法人日本薬学会の論文
1978-02-25