螢光滴定による体液リパーゼ活性の微量測定

概要

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An improved method for microassay of lipase activity is described. The method is an application of fluorometric titration to fatty acids. Fatty acids liberated from an olive oil emulsion on treatment with lipase at 37° for 5-20 min are extracted with Dole's extraction mixture and an aliquot of the heptane solution of the acids, containing at least 0.1-2.0μeq, is titrated with 0.01_N isopropanolic sodium hydroxide using isopropanol solution of umbelliferone as a fluorescent indicator. Constancy of the reaction rate is obtained by 40-min incubation. Short incubation time, precision, and sensitivity make this method suitable for the assay of lipase in body fluid. Preliminary application of this method to some samples of serum, bile, and pharmaceutical preparations is mentioned.
公益社団法人日本薬学会の論文
1975-02-25