骨髄細胞コロニー形成に対するブタ脾臓中の低分子量阻害因子(非ペプチド)の精製及び性質

概要

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Aqueous extracts prepared from porcine spleens were found to inhibit colony growth in agar cultures of mouse bone marrow cells stimulated by L-cell conditioned medium (LCM). The inhibitory activity in the extracts was heat stable and was not ether extractable. Preincubation of the spleen extracts with LCM failed to inhibit colony formation in subsequent agar cultures. This suggests that if inhibitor-colony stimulating factor (CSF) complexes exist in culture system, they must be readily dissociable and/or inhibitors can not bind to the active site (s) of CSF. Two highly purified inhibitors were obtained from the extracts by a combination of gel filtration, silica gel column chromatography, paper electrophoresis and cellulose thin layer chromatography. Analytical procedures suggested that these inhibitors had an apparent molecular weight of 300 and 400,respectively, and were not peptides.
社団法人日本薬学会の論文
1984-10-25