イソタコフォレシスによるローヤルゼリー中の10-Hydroxy-Δ^2-decenoic Acidの定量

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概要

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• The working conditions for rapid analysis of 10-hydroxy-Δ^2-decenoic acid (I) in royal jelly by means of capillary tube isotachophoresis were studied. The leading electrolyte composed of 0.01 M hydrochloric acid in 30% methanolic solution was adjusted to pH 5 with L-histidine, while the terminal electrolyte of 0.01 M 2 (N-morpholino) ethanesulfonic acid monohydrate in 30% methanolic solution was adjusted to pH 7 with tris (hydroxymethyl) aminomethane. I was determined within 10 minutes without any interference. Both sebacic acid and 10-hydroxydecanoic acid co-existing as side fractions were also identified. This method is simple and fast as well as sensitive and quantitative, eliminating the need of pretreatment of the sample.

• 1981-02-25

著者

• 後藤 一

  ゼリア新薬工業株式会社製剤研究所

• 武藤 泰章

  ゼリア新薬工業株式会社中央研究所

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