食品の保存料, 人工甘味料などの衛生化学的研究(第12報) : 食品中の水溶性着色料の検出

概要

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The application of DEAE-Sephadex to the problem of separating certifiable acid dyes in foods and the detection of the dyes by thin-layer chromatography are described. The dyes were able to be transfered from a food to DEAE-Sephadex by only shaking mixture of the both, even when they were bound to cellulosic of proteinous substrates. The dyes were eluted from a column of the dyes-bound DEAE-Sephadex with the equivolume mixture of 2N sulfuric acid isopropyl alcohol. The dyes concentration in eluate was satisfactory for its detection by silicagel and thin-layer chromatography. The solvent systems for the chromatography were composed of ethyl acetate-methyl alcohol-aqueous ammonia (4.5 : 1 : 0.7,or 3.3 : 1 : 1). These systems were most recomendable for the separation of the tested dyes in this experiment ; the former was suitable for the separation of the xanthen dyes and the later of the others.
公益社団法人日本薬学会の論文
1969-04-20