高感度Amesテストによる都市大気浮遊粒子状物質の変異原性の粒径別分析

概要

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The mutagenicity of airborne particulates in Tokyo was evaluated by the Salmonella microsuspension procedure. Airborne particles were collected in 9 collection stages according to their aerodynamic diameter and tested for the mutagenicity with S. typhimurium strain TA 98. Because the microsuspension procedure is highly sensitive, it was possible to detect the mutagenic activity from only 10 μg of the particles of less than 2.5 μm in diameter. Moreover we could detect the mutagenic activity of the particles ranging from 2.5 to 10 μm, which had been difficult to be detected by the original Ames test. The particles collected in winter showed higher activity than those in summer. The mutagenic activity was high in the particles of less than 0.4 μm and of about 1 μm in winter. In the same season, the specific activity for the mutagenicity of the particulates did not differ in the sampling area. The concentration of polyaromatic hydrocarbons in airborne particulates correlated with the mutagenicity of those in every collection stage, and it was suggested that mutagens in particulate matter behave as polyaromatic hydrocarbons.
公益社団法人日本薬学会の論文
1993-04-30