Isolation of Dichloromethane-Degrading Bacteria from Drainage Water

概要

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The biodegrading ability of drainage water from research laboratories to dichloromethane (DCM) and chloroform (CF) was surveyed. When DCM was used as a sole carbon source in a synthetic mineral salt medium, some water samples showed ability to degrade DCM, and DCM-degrading bacteria were isolated from them, whereas no samples showed CF degradation activity. Two isolates, strain P3310,a Flavimonas sp., and strain G31,a Chryseobacterium sp., were used for further investigations. Both strains were able to use DCM as a carbon source for growth and also grow in complex media containing other carbon sources, suggesting they werefacultative methylotroph. Both strains needed 6 days at 30℃ to completely degrade 200 mg/l of DCM with the first isolated cells, but this was shortened to 2 days with the first subculture, suggesting they were acclimatized. Although the DCM-degrading activity of strain G31 was inhibited by addition of other carbon sources such as peptone or glucose, that of strain P3310 was not affected. Thus, strain P3310 may be a useful candidate for bioremediation to eliminate DCM from drainage.
公益社団法人日本薬学会の論文
2000-06-01