Lipid Peroxidation in Egg Phosphatidylcholine Liposomes : Comparative Studies on the Induction Systems Fe^<2+>/Ascorbate and Fe^<3+>-Chelates/Xanthine-Xanthine Oxidase
スポンサーリンク
概要
- 論文の詳細を見る
Two typical systems of lipid peroxidation in egg yolk phosphatidylcholine (egg PC) liposomes were compared : an enzymic system involving superoxide (O^-_2) generated by xanthine (X), xanthine oxidase (XO) and Fe^<3+>-chelates (Fe^<3+>-ADP and Fe^<3+>-EDTA), and a non-enzymic system involing ascorbic acid (AsA) and Fe^<2+>. These two systems exhibited a different pH-dependence : the rate in the enzymic system was maximal at pH 8-8.5,wheareas that in the non-enzymic system was high below pH 7.4 and low above pH 7.6. The rates of lipid peroxidation differed with the membrane charge, and this charge-dependent phenomenon differed in the two peroxidation systems : in the Fe^<3+>-chelates/X-XO-system, the rate was slow in neutrally charged egg PC liposomes and rapid in egg PC liposomes containing negatively charged dicetylphosphate (DCP) or positively charged stearylamine (SA), whereas in the Fe^<2+>/AsA-system, the rate was rapid in neutral egg PC liposomes but no lipid peroxidation occurred in egg PC liposomes charged with DCP or SA. The decomposition rate of the hydroperoxide of PC (PC-OOH) incorporated into dimyristoyl-phosphatidylcholine (DMPC) liposomes differed depending on the membrane charge in the two systems and this charge-dependence of the rates correlated well with that of the initiation rate of lipid peroxidation dependent on membrane charge. In the Fe^<2+>/AsA-system, lipid peroxidation depended on the endogenous presence of PC-OOH, and the amounts of PC-OOH required for initiation of the reaction differed depending on the membrane charge. However, in the Fe^<3+>-chelates/X-XO-system, lipid peroxidation occurred very slowly in the absence of PC-OOH, but rapidly in its presence.
- 1996-05-15
著者
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Fukuzawa Kenji
Department of Micro Nano Systems Engineering, Nagoya University
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Fukuzawa K
Department Of Health Chemistry Faculty Of Pharmaceutical Sciences Tokushima University
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IEMURA Masahito
Department of Health Chemistry, Faculty of Pharmaceutical Sciences, Tokushima University
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TOKUMURA Akira
Department of Health Chemistry, Faculty of Pharmaceutical Sciences, Tokushima University
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Iemura Masahito
Department Of Health Chemistry Faculty Of Pharmaceutical Sciences Tokushima University
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Tokumura A
Univ. Tokushima Tokushima Jpn
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Tokumura Akira
Department Of Health Chemistry Faculty Of Pharmaceutical Sciences Tokushima University
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Fukuzawa Kenji
Department Of Micro Nano System Engineering Graduate School Of Engineering Nagoya University
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FUKUZAWA Kenji
Department of Health Chemistry, Faculty of Pharmaceutical Sciences, Tokushima University
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