Platelet-activating Factor (PAF)-like Phospholipids Formed during Peroxidation of Phosphatidylcholines from Different Foodstuffs

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概要

• 論文の詳細を見る

• Previously, we reported that induction of peroxidation of synthetic phosphatidylcholines (PCs) containing a polyunsaturated fatty acid by Fe^<2+>-EDTA in the presence of ascorbate resulted in the formation of four types of PCs with an sn-2-oxidatively fragmented acyl group, which had platelet-aggregating activity due to interaction with platelet-activating factor (PAF) receptors. These PCs were compounds with a short-chain monocarboxylate, ω-hydroxymonocarboxylate, dicarboxylate, and dicarboxylate semialdehyde residue, respectively. In this study, we investigated the PAF-like lipids formed during peroxidation of PCs from hen egg yolk, salmon roe, sea urchin eggs, and krill in an FeSO_4/EDTA/ascorbate system. The platelet-aggregating activities of these oxidized PCs were all inhibited by FR-900452, an antagonist of PAF. The activity of oxidized krill PC, which was equivalent of 89.8±8.8 pmol 16:0-PAF/μmol of starting PC, was about 5 times those of oxidized PCs from salmon roe and sea urchin eggs, and about 50 times that of oxidized hen egg yolk PC. The PAF-like phospholipids that had different combinations of long-chain alkyl or acyl groups with one of the above four types of short-chain acyl groups were identified by gas chromatography-mass spectrometry. The results indicated that foodstuffs that are rich in 1-O-alkyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine are potential sources of compounds with high PAF-like activity formed by deleterious lipid peroxidation.

• 社団法人日本農芸化学会の論文
• 1995-08-23

著者

• TSUKATANI HIROAKI

  Faculty of Pharmaceutical Sciences, University of Tokushima

• Tanaka Tamotsu

  Faculty Of Pharmaceutical Sciences The University Of Tokushima

• TOKUMURA AKIRA

  Faculty of Pharmaceutical Sciences, Tokushima University

• Tokumura A

  Univ. Tokushima Tokushima Jpn

• Tokumura Akira

  Faculty Of Pharmaceutical Sciences The University Of Tokushima

• Tsukatani H

  Faculty Of Pharmaceutical Sciences The University Of Tokushima

• Tsukatani Hiroaki

  Faculty Of Pharmaceutical Sciences Tokushima University

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• Lipid Peroxidation in Egg Phosphatidylcholine Liposomes : Comparative Studies on the Induction Systems Fe^/Ascorbate and Fe^-Chelates/Xanthine-Xanthine Oxidase
• COMPARISON OF ANTISPASMODIC EFFECT OF SYNTHETIC LYSOLECITHINS WITH VARIOUS FATTY ACID MOIETIES ON GUINEA PIG ILEUM
• CARDIOVASCULAR EFFECT OF "PERITONEAL DIALYSATE-DEPRESSOR-I", A SHORT-ACTIVE HYPOTENSIVE PHOSPHOLIPID IN DOG PERITONEAL DIALYSATE
• α-Tocopheryl Succinate Activates Protein Kinase C in Cellular and Cell-Free Systems
• Phorbol Myristate Acetate Stimulates Degradation of a Structural Analogue of Platelet-Activating Factor to a Neutral Lipid in Human Leukemic K562 Cells : Relevance to the Release of Lipids(Biochemistry/Molecular Biology)
• Platelet-activating Factor (PAF)-like Phospholipids Formed during Peroxidation of Phosphatidylcholines from Different Foodstuffs
• ACTION OF DEPRESSOR-I, A HYPOTENSIVE PHOSPHOLIPID FROM BOVINE BRAIN, ON SYSTEMIC AND ARTERIAL BLOOD PRESSURES OF VARIOUS SPECIES

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