Quantification of a Very Low Level of Serum Human Immunodeficiency Virus Type 1 (HIV-1) RNA Using Competitive PCR at Intervals and the Clinical Course
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概要
- 論文の詳細を見る
Quantification of human immunodeficiency virus type 1 (HIV-1) genomic RNA levels below the detection limit (400 copies/ml) of commercially available quantification kits is possible by increasing the amount of serum samples using competitive PCR conditions. We evaluated disease prognosis in patiets without symptoms for a long period after infection (long-term non-progressor) and patients in whom the virus was controlled to a low level by administration of anti-HIV drugs. For 102 of the 414 serum samples stored at -80℃ which showed HIV-1 RNA below 400 copies/ml by competitive PCR using 100 to 200 μl sera, an increase in the sample volume to 500-2000 μl, extraction of HIV-1 RNA, and quantitative detection by competitive PCR was performed. Follow-up quantification of serum HIV-1 RNA from 4 patients indicate that this method is useful in assessing prognosis in the early stage of the disease in patients without symptoms for a long period after infection (long-term non-progressor) and patients in whom the virus in the serum was controlled by administration of anti-HIV drugs to below the detection limit of commercially available quantification kits. Quantification of low level serum HIV-1 RNA was also considered useful as a parameter of treatment.
- 社団法人日本薬学会の論文
- 2000-03-01
著者
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内藤 博敬
静岡県大・環境科学研・環境微生物
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内藤 博敬
静岡県立大学大学院生活健康科学研究科
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Naitou Hirotaka
Graduate School Of Nutritional And Environmental Sciences And Global Coe Program Univ. Of Shizuoka
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Naitou H
Laboratory Of Environmental Microbiology Institute For Environmental Sciences University Of Shizuoka
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Naitou Hirotaka
Graduate School Of Nutritional And Environmental Sciences University Of Shizuoka:global Coe Universi
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Naitou Hirotaka
Laboratory Of Environmental Microbiology Graduate School Of Nutritional And Environmental Sciences U
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NAITOU Hitoraka
Laboratory of Environmental Microbiology, Institute for Environmental Sciences, University of Shizuo
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MIMAYA Jun-ichi
Division of Hematology and Oncology, Shizuoka Children's Hospiral
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HORIKOSHI Yasuo
Division of Hematology and Oncology, Shizuoka Children's Hospiral
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MORITA Tamotsu
Laboratory of Environmental Microbiology, Institute for Environmental Sciences, University of Shizuo
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Morita T
Laboratory Of Environmental Microbiology Institute For Environmental Sciences University Of Shizuoka
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Morita Tamotsu
Laboratory Of Environmental Microbiology Graduate School Of Nutritional And Environmental Sciences U
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Mimaya Jun-ichi
Department Of Hematology/oncology Shizuoka Children's Hospital
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MIMAYA Jun-ichi
Pediatrics
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Horikoshi Yasuo
Department Of Hematology/oncology Shizuoka Children's Hospital
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Horikoshi Yasuo
Division Of Hematology And Oncology Shizuoka Children's Hospital
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Naitou Hirotaka
Graduate School Of Nutritional And Environmental Sciences And Global Coe Program University Of Shizu
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Naitou Hirotaka
Institute For Environmental Sciences University Of Shizuoka
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