Analysis of the Distruption Mutant of the Oscillin Homolog Gene of Dictyostelium discoideum

概要

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A homolog of oscillin, the Ca^<2+> oscillation-inducing factor of the hamster, was identified from the cellular slime mold Dictyostelium discoideum and designated Dd-oscillin. In the developmental stages of D. discoideum, the gene is expressed at the prestalk region which contains higher concentration of cytosolic Ca^<2+> than the prespore region. The Dd-oscillin null strain aggregated but did not develop further when the cells were plated on non-nutrient agar at a density of 1.5×10^6 cells/cm^2,showing that the Dd-oscillin gene is important for development. Since the null cells carrying the hamster oscillin gene formed fruiting body, the hamster oscillin was the homolog of Dd-oscillin as far as function is concerned. In addition, the null cells formed fruiting body in the presence of 2,5-di(tert-butyl)-1,4-hydroquinone (BHQ : a specific inhibitor of Ca^<2+>-ATPase activity in the endoplasmic reticulum). These results suggest that Dd-oscillin will increase cytosolic Ca^<2+> in the cells and promote further development.
公益社団法人日本薬学会の論文
1999-09-15