Identification of Three Hydroxysteroid Sulfotransferase Isoenzymes in the Rat Liver
スポンサーリンク
概要
- 論文の詳細を見る
Isolation of several hydroxysteroid sulfotransferase (HS-ST) cDNAs from the rat liver cDNA library has demonstrated the possible expression of these HS-ST isoforms in rat liver. We devised a method to detect unequivocally ST-20 and ST-40 mRNAs by a reverse transcription-polymerase chain reaction, using specific primers. ST-40 mRNA was expressed only in liver, but ST-20 mRNA was present predominantly in the liver and slightly in extrahepatic tissues. On chromatofocusing, expressed ST-20 or ST-40 enzymes were eluted at approx.pH8.2 and 5.7-4.7 or at pH6.4-5.4,respectively. Chromatofocusing of adult female rat liver cytosols resolved HS-ST isoenzymes in a broad range of pH, and ST fractions A, B, C, D and E were eluted at approx. pH 8.2,7.6,7.5-6.8,6.2 and 6.1-5.5,respectively. After PAP-agarose affinity column chromatography and SDS-polyacrylamide gel electrophoresis (PAGE), their N-terminal amino acid sequences were determined. ST isoenzymes present in fractions B and E showed identical N-terminal amino acid sequences with those of ST-21 and ST-20,respectively, whereas the ST isoenzymes present in fractions C and D had the same N-terminal amino acid sequence as those of ST-40 (and/or ST-41). The results demonstrated the presence of at least three HS-ST isoenzymes in adult female rat liver.
- 公益社団法人日本薬学会の論文
- 1998-01-15
著者
-
TAMURA Hiro-omi
Kyoritsu College of Pharacy
-
MATSUI Michio
Kyoritsu College of Pharmacy
-
Takahashi Mie
Kyoritsu College Of Pharmacy
-
KONDO Sayuri
Kyoritsu College of Pharmacy
-
KOBAYASHI Kie
Kyoritsu College of Pharmacy
関連論文
- Mg^ Binding and Catalytic Function of Sphingomyelinase from Bacillus cereus
- A Novel Sulfotransferase Abundantly Expressed in the Dauer Larvae of Caenorhabditis elegans
- Cloning, Expression, and Characterization of Cytosolic Sulfotransferase Isozymes from Drosophila melanogaster
- A New Mutant Strain of Gunn-LA Wistar Rats with Genetic Deficiencies in Bilirubin and Androsterone Uridine Diphosphate-Glucuronosyltransferases
- A COMPARISON OF URIDINE DIPHOSPHATE-GLUCURONOSYL-TRANSFERASE AND OTHER DRUG METABOLIZING ENZYME ACTIVITIES BETWEEN TWO MUTANT STRAINS OF WISTAR RATS WITH A GENETIC DEFICIENCY IN BILIRUBIN OR ANDROSTERONE GLUCURONIDATION
- Cytochrome P450 (CYP) Expression in Human Myeloblastic and Lymphoid Cell Lines
- Expression Profiling of Sulfotransferases in Human Cell Lines Derived from Extra-Hepatic Tissues
- Region- and Isoform-Specific Expression of Hydroxysteroid Sulfotransferases in Rat Brain
- Identification and Purification of Sulfotransferases for 20-Hydroxysteroid from the Larval Fat Body of a Fleshfly, Sarcophaga peregrina(Biochemistry & Molecular Biology)
- Epigallocatechin Gallate(EGCG)Inhibits the Sulfation of 1-Naphthol in a Human Colon Carcinoma Cell Line, Caco-2
- Inhibitory Effects of Green Tea and Grape Juice on the Phenol Sulfotransferase Activity of Mouse Intestines and Human Colon Carcinoma Cell Line, Caco-2
- Oxidative Stress Induces GSTP1 and CYP3A4 Expression in the Human Erythroleukemia Cell Line, K562 (Biochemistry/Molecular Biology)
- Changes in the Gene Expression Patterns of the Cytochrome P450s in Selenite-induced Cataracts in the Rat
- The Effects of Coffee on Conjugation Reactions in Human Colon Carcinoma Cells(Toxicology)
- Effects of Gonadal Hormones on the Zonal Expression of Rat Hepatic Phenol Sulfotransferases
- RAT LIVER SULFOTRANSFERASES : EFFECTS OF GONADAL HORMONES AND OTHER FACTORS ON ENZYME ACTIVITIES
- Identification and Localization of Two Hydroxysteroid Sulfotransferases in the Human Brain
- Sulfortransferases in a Human Colon Carcinoma Cell Line, Caco-2
- Molecular Cloning, Expression and Characterization of a Phenol Sulfotransferase cDNA from Mouse Intestine
- Effect of Herbal Teas on Conjugation Reactions in a Human Colon Carcinoma Cell Line, Caco-2
- Cloning, Expression, and Characterization of Cytosolic Sulfotransferase Isozymes from Drosophila melanogaster
- Cytochrome P450 Expression in Rat Ocular Tissues and Its Induction by Phenobarbital
- HETEROGENEITY OF RAT LIVER SULFOTRANSFERASES
- GENETIC DEFECT OF URIDINE DIPHOSPHATE-GLUCURONOSYLTRANSFERASE ISOENZYME
- Identification of Three Hydroxysteroid Sulfotransferase Isoenzymes in the Rat Liver
- MULTIPLE FORMS AND A DEFICIENCY OF URIDINE DIPHOSPHATE-GLUCURONOSYLTRANSFERASES IN WISTAR RATS
- Effects of Phenolic Environmental Estrogens on the Sulfotransferase Activity of the Mouse Intestine and a Human Colon Carcinoma Cell Line, Caco-2
- Cloning and Expression of a Novel Sulfotransferase with Unique Substrate Specificity from Bombyx mori
- Sulfoconjugation of Bisphenol A in a Human Neuroblastoma Cell Line, NB-1