Molecular Cloning, Expression and Characterization of a Phenol Sulfotransferase cDNA from Mouse Intestine
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概要
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A sulfotransferase (ST) cDNA was isolated from a mouse intestinal cDNA library using a probe which was generated by reverse transcription (RT)-PCR based on the conserved amino acid sequences of the ST molecules. The isolated cDNA (1.1 kb) contained an 858 bp open reading frame encoding a 286 amino acid polypeptide with molecular weight of 33439. The deduced amino acid sequence shares 55.1% and 40.2% identiry with mouse liver aryl/phenol (mSTp1) and alcohol (mSTa1 or mSTa2) STs, respectively, and it is highly similar to those of rat and human liver phenol ST (P-ST) isozymes, ST1B1 (87.8%) and ST1B2 (71.0%), respectively. RT-PCR analyses showed abundant expression of the P-ST mRNA in the intestine as well as in the liver in the mouse tissues examined (brain, heart, intestine, kidney, liver and lung) of both sexes. E. coli-expressed enzyme is capable of catalyzing the sulfation of 2-naphthol at K_m=3.3 μM and V_<max>=3.33 nmol/min/mg protein and also showed sulfation activity for L-3,4-dihydroxyphenylalanine (L-DOPA) and dopamine. Among food constituents tested, tannic acid and epigallocatechin gallate strongly inhibited the P-ST activity in vitro.
- 1999-03-15
著者
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Mikoshiba Katsuhiko
Riken Brain Science Institute
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Mikoshiba Katsuhiko
The Institute Of Medical Science The University Of Tokyo
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Tamura H
Seikagaku Corporation
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MATSUI Michio
Kyoritsu College of Pharmacy
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MIYAWAKI Atsushi
The Institute of Medical Science, The University of Tokyo
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YONESHIMA Hiroyuki
The Institute of Medical Science, The University of Tokyo
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Yoneshima Hiroyuki
The Institute Of Medical Science The University Of Tokyo
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Miyawaki Atsushi
Cell Function Dynamics Riken Brain Science Institute
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Miyawaki Atsushi
The Institute Of Medical Science The University Of Tokyo:(present Address)cell Function And Dynamics Brain Research Institute
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