Effect of Astragali Radix Extract on Lipopolysaccharide-Induced Inflammation in Human Amnion

概要

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The effects of Astragali radix extract on interleukin(IL)-6 and tumor necrosis factor(TNF)-α productions, prostaglandin E_2(PGE_2)biosynthesis, and leukotriene C_4(LTC_4)production from lipopolysaccharide(LPS)-stimulated human amnion cells were investigated. Anion cells produced detectable amounts of both IL-6 and TNF-α under LPS-stimulated conditions, Astragalus extract inhibited the production of IL-6. However, TNF-α production was not inhibited by the extract on L929 cytotoxicity assay. Treatment of amnion cells with LPS for up to 24 h resulted in an increase in PGE_2 release in a concentration- and time-dependent manner. The extract(150 mg/ml)significantly inhibited the output of PGE_2 by amnion cells(p<0.01). The arachidonate lipoxygenase metabolite(LTC_4)was increased by LPS treatment of amnion cells. Astragalus extract(30 mg/ml)inhibited LTC_4 production by approximately 65% throughout the culture period. These results suggest that Astragali radix extract may have a role in inhibiting bacterial infection-associated preterm labor by suppressing the productions of IL-6, PGE_2, and LTC_4 human amnion cells.
公益社団法人日本薬学会の論文
2002-01-01