Protective Effect of Moutan Cortex Extract on Acetaminophen-Induced Cytotoxicity in Human Chang Liver Cells

概要

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The aim of this study was to investigate the effect of Moutan Cortex on acetaminophen (AAP)-induced toxicity in human Chang liver cells. Cells were incubated with AAP (0-30 m_M) to evaluate the drug's ability to reduce cytoviability. For the cells treated with 10, 20 and 30 m_M AAP, LDH leakage was 39.8%, 49.0% and 57.6%, respectively. Administration of Moutan Cortex reduced cytotoxicity in a dose-dependent manner. Glutathione (GSH) concentration in human liver cells decreased significantly after exposure to 20 (p<O.05) and 30 m_M (,p<O.O1) AAP, and increased (p<O.OS) if incubated with AAP and Moutan Cortex. The ability of AAP to inhibit mitochondrial function and its counteraction by Moutan Cortex was also evaluated. Moutan Cortex showed dose-dependent increases in MTT metabolism and ATP levels in AAP-treated cells. The DNA content of AAP-treated cells increased with the treatment of Moutan Cortex. These observations demonstrate that Moutan Cortex may significantly attenuate AAP-induced toxicity. It can be considered a cytoprotective agent in this in vitro model of drug toxicity.
公益社団法人日本薬学会の論文
2002-11-01