Effect of Acute-phase Proteinase Inhibitors on Chemotaxis of Rat Polymorphonuclear Leukocytes in Vitro

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Rat serum obtained at 24 h after subcutaneous injection of carrageenin significantly suppressed chemotaxis of rat polymorphonuclear leukocytes (PMNs) in vitro. α_2 Acute-phase macroglobulin (α_2APM), α_1 proteinase inhibitor (α_1PI) and cysteine-proteinase inhibitors (CPIs) are present at high concentration in the 24-h serum and known as acute-phase reactants in rats. These acute-phase proteinase inhibitors were purified from inflamed rat serum or exudate and their effect on PMN chemotaxis was studied by Boyden's method in vitro. α_2APM (4 mg/ml) significantly suppressed PMN chemotaxis while α_1M was without effect, though both α_2APM and α_1M had a similar anti-proteinase activity. The results suggest that α_2APM suppressed PMN chemotaxis through the mechanism unrelated to its anti-proteinase activity. On the other hand, α_1PI (1 and 3 mg/ml) slightly but significantly suppressed PMN chemotaxis, whereas CPI-1 and CPI-2 had no inhibitory effect. These results suggest that α_2APM and α_1PI play a role in suppression of PMNs infiltration into the inflammatory site in the late-phase of acute inflammation.
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