PREPARATION AND ANTIGENIC PROPERTIES OF DEHYDROEPI-ANDROSTERONE SULFATE-BOVINE SERUM ALBUMIN CONJUGATES HAVING A LINKAGE THROUGH THE POSITION IN RING D
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概要
- 論文の詳細を見る
The preparation and antigenic properties of two different types of dehydroepiandrosterone sulfate-bovine serum albumin (BSA) conjugates in which the hapten is linked to the carrier protein through the C-17 or C-15 position have been described. Antibodies raised against these antigens in rabbits possessed the high affinity (K_a=1.43×10^9-2.20×10^9M^<-1>) to dehydroepiandrosterone sulfate. Antiserum elicited by immunization with the hapten-15β-BSA conjugate was specific to dehydroepiandrosterone sulfate, exhibiting no significant cross-reactivities for other sulfated steroids and no cross-reactions for free steroids and their glucuronides with an exception of 16α-hydroxydehydroepiandrosterone 3-sulfate (16.8%).
- 公益社団法人日本薬学会の論文
著者
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Shimada Kazutake
Pharmaceutical Institute Tohoku University
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Ohta Hiroko
Pharmaceutical Institute Tohoku University
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Nambara Toshio
Pharmaceutical Institute Tohoku University
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Sawada Yoshiko
Pharmaceutical Institute Tohoku University
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NUMAZAWA MITSUTERU
Pharmaceutical Institute, Tohoku University
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Numazawa Mitsuteru
Pharmaceutical Institute Tohoku University
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