PREPARATION OF SPECIFIC ANTISERA TO ESTRIOL 16-GLUCURONIDE BY IMMUNIZATION WITH STEROIDS ATTACHED TO PROTEIN THROUGH POSITIONS IN RING A
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概要
- 論文の詳細を見る
Antigenic properties of three new types of estriol 16-glucuronide-bovine serum albumin (BSA) conjugates in which the steroid hapten was linked to a carrier protein through the C-2 or C-4 position have been investigated. All antibodies elicited by immunization with these antigens showed the high affinity (K_a=1.41×10^9-3.50×10^9M^-) and the excellent specificity to estriol 16-glucuronide. No significant cross-reactivity was observed with other glucuronides, sulfates and free steroids except for estriol 17-glucuronide (0.78-1.34%). These antisera appear to be useful for direct radioimmunoassay of estriol 16-glucuronide in biological fluids without prior hydrolysis and chromatographic purification.
- 公益社団法人日本薬学会の論文
著者
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Nambara Toshio
Pharmaceutical Institute Tohoku University
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Ohkubo Tadashi
Pharmaceutical Institute Tohoku University
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Numazawa Mitsuteru
Pharmaceutical Institute Tohoku University
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TANAKA TOUICHI
Pharmaceutical Institute, Tohoku University
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Tanaka Touichi
Pharmaceutical Institute Tohoku University
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NAMBARA TOSHIO
Pharmaceutical Institute, Tohoku University
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