EFFECT OF ADENOSINE 3', 5'-MONOPHOSPHATE ON GROWTH AND SEVERAL FUNCTIONS OF CULTURED MASTOCYTOMA P-815 CELLS
スポンサーリンク
概要
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Growth-inhibited mouse mastocytoma P-815 cells at stationary phase contained more histamine, serotonin and adenosine 3', 5'-monophosphate (cAMP), and higher activities of histidine decarboxylase and adenylate cyclase than the cells during exponential growth. The elevation of endogenous cAMP levels induced by several growth-inhibiting agents such as N^6,O^<2'>-dibutyryl cAMP (Bt_2cAMP), prostaglandin E_1,AMP and 2-chloroadenosine stimulated several functions characteristic of mastocytoma P-815 cells in culture, elevating the synthesis of histamine and serotonin, the activity of chymotrypsin-like protease, and the incorporation of [^<35>S] sulfate into acidic glycosaminoglycans. 1-Methyl-3-isobutyl-xanthine (MIX), a potent inhibitor of cAMP phosphodiesterase, potentiated stimulatory effect of these agents. The results indicate that cAMP regulates the growth and functions of mastocytoma P-815 cells. [^<35>S]-Sulfated acidic glycosaminoglycans synthesized in cells at stationary phase or in cells treated with Bt_2cAMP plus MIX mainly localized in the 3000-10000×g sedimentable fraction of cell homogenates, and had a molecular weight of 200000 to 400000 based on gel filtration. This acidic glycosaminoglycan was resistant to condroitinase ABC and the heparindegrading enzyme present in the 20000×g sedimentable fraction of the cells, and was identified as a highly sulfated macromolecular heparin based on behaviors on DEAE-cellulose column and on acidic electrophoresis. Cycloheximide suppressed the stimulatory effect of Bt_2cAMP on the synthesis of histamine and [^<35>S]-sulfated acidic glycosaminoglycan.
- 公益社団法人日本薬学会の論文
著者
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ICHIKAWA Atsushi
Department of Hematology, Tajimi Prefectural Hospital
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Esumi Kimio
Department Of Health Chemistry Faculty Of Pharmaceutical Sciences Kyoto University
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Yatsunami Kimio
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Kyoto University
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Ichikawa A
Kyoto Univ. Kyoto Jpn
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Ichikawa Atsushi
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Kyoto University
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NEGISHI Manabu
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kyoto University
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TAKAGI MASAKI
Department of Health Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University
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TOMITA KENKICHI
Department of Health Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University
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Takagi Masaki
Department Of Health Chemistry Faculty Of Pharmaceutical Sciences Kyoto University
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Negishi Manabu
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Kyoto University
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Tomita Kenkichi
Department Of Health Chemistry Faculty Of Pharmaceutical Sciences Kyoto University
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