A Simple Purification Procedure for α-Fetoprotein by Immunoadsorbent Column Chromatography
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概要
- 論文の詳細を見る
Human α-fetoprotein was purified from human cord serum by employing an immunoadsorbent column, consisting of Sepharose 4B coupled with the antibody to human α-fetoprotein. By using 0.2 M Na_2CO_3 solution (pH 11.6) as an eluent, a large amount of α-fetoprotein was obtained from the immunoadsorbent column in high purity. After gel filtration on Sephadex G-150,the purified α-fetoprotein was demonstrated to be homogeneous by polyacrylamide gel electrophoresis, SDS-gel electrophoresis and two-dimensional immunoelectrophoresis.
- 公益社団法人日本薬学会の論文
- 1982-09-25
著者
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渡辺 義人
Gifu Pharmaceutical University
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足立 哲夫
Gifu Pharmaceutical University
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伊藤 吉将
Gifu Pharmaceutical University
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杉浦 衛
Gifu College Of Pharmacy
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平野 和行
Gifu College Of Pharmacy
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