Studies on Bile-sensitive Lipase. VIII. Product Inhibition of Mucor Lipase and Role of Bile Salts and Ca^<2+> to the Inhibition

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In order to clarify the effect of the products formed during lipolysis by Mucor lipase, some experiments were done. The lipase was inhibited by soaps of long-chain fatty acids above C_<12>, while the lipase was little affected by soaps of C_4-C_8 fatty acids, glycerin and monoglycerides. Long-chain fatty acids in very low concentration, less than 5 μmoles, acted as a competitive inhibitor. Although diffusion of oleic acid from the interface was arrested by laurate and oleate because of the accumulation of the salts at the interface, by the addition of bile salt to the system diffusion of oleic acid was promoted. Bile salts reduced inhibition by long-chain fatty acids, consequently the initial rate of the reaction did not reduce for some time. Ca^<2+> also prevented the inhibition by removing the fatty acids into the oil phase after the formation of Ca-soaps.
公益社団法人日本薬学会の論文
1970-04-25