Kinetics of Digestive Enzyme Stability in the Solid State. II. Quantitative Prediction of Enzyme Inactivation

概要

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The procedure for predicting digestive enzyme stability in the solid state was further investigated by utilizing the Weibull distribution function, which needs two mathematically meaningful parameters, m and k. These parameters could be estimated by graphic calculation. If the parameter m is independent of temperature, the Arrhenius plots of (1/m) 1nk versus 1/T are linear since k^<1/m> is proportional to the inactivation rate constant. The parameter k could be estimated by extrapolating to the desired temperature in the Arrhenius plots and the activation energy could be determined from the slope of this line. These parameters, m and k, made it possible to predict the inactivation ratio of enzymes in the solid state. By comparing the predicted value of the inactivation ratio with the observed value under controlled conditions, it was shown that the proposed method is accurate and useful for studies on enzyme stability in the solid state.
公益社団法人日本薬学会の論文
1981-07-25