Studies on the Metabolic Fate and the Pharmacokinetics of 5-n-Butyl-1-cyclohexyl-2,4,6-trioxoperhydropyrimidine (BCP) in Man. III. Gas-Liquid Chromatographic Determination of BCP and Its Metabolites

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An investigation was carried out on the gas-liquid chromatographic determination of 5-n-butyl-1-cyclohexgl-2,4,6-trioxoperhydropyrimidine (BCP) and its metabolites, i.e., 1-cyclohexyl-5-(3-hydroxybutyl)-2,4,6-trioxoperhydropyrimidine (II) and 5-n-butyl-1-(4-hydroxycyclohexyl)-2,4,6-trioxoperhydropyrimidine (IV) in human urines. Neopentyl glycol succinate (NGS) 2% on Gas Chrom P was selected as the liquid stationary phase and a hydrogen flame ionization detector was employed. By trimethylsilylation of the chloroform extract from a urine, BCP and IV were determined simultaneously with phenobarbital as the internal standard, for IV was subjected trimethylsilylation, while BCP was not affected by the treatment. The metabolite II was determined selectively by heating it in an acid medium to convert into 3-cyclohexyl-7-methyl-1,2,3,4,6,7-hexahydro-5-H-pyrano (2,3-d) pyrimidine-2,4-dione (XV). Cyclobarbital or phenylbutazone was used as the internal standard in this case.
社団法人日本薬学会の論文
1971-03-25